Directive 2002/32 / EC sets out requirements for upper limits on unwanted substances in animal feed, such as organochlorine compounds and arsenic.
EN 15741: 2020 Animal Feed: Sampling and Analytical Methods-Measurement of OCP and PCB by GC-MS
Organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) are two groups of highly toxic organochlorine pollutants present in the environment and are incorporated into the food chain by contamination of animal diets that need to be monitored. there is a possibility.
EN 15741: 2020 describes how to measure specific OCPs and PCBs in animal feeds and oils using gas chromatography in combination with a mass spectrometer detector (GC-MS) in EN 15741: 2009. I will replace it.
Technical changes have been made to EN 15741: 2009 to determine non-dioxin-like PCBs (ndl-PCBs) and include two new approaches: (1) Original extraction and cleanup steps are more sensitive Combined with high detection techniques (2) Extraction and cleanup are modified to increase the size of the test area, but still use the original detection techniques.
This method has been validated by collaborative testing of 12 categories of OCPs and PCBs, and some of their isomers and degradation products that vary widely in the range of 1-300 µg / kg. The specified OCP quantification limit (LOQ) is 6 to 29 µg / kg, and 0.5 to 1 µg / kg can be achieved for ndl-PCB.
This standard shows that test results can only be considered for screening purposes for certain matrix-analytical type combinations with inadequate validation information.
EN 17362: 2020 Animal Feed: Sampling and Analytical Methods-Measurement of Pentachlorophenol (PCP) in Feed Materials and Compounds Feed by LC-MS / MS
Pentachlorophenol (PCP) is an organochlorine compound previously used as a disinfectant for the treatment of fibers and wood. It is no longer manufactured in the EU and is found in the environment as a persistent toxic pollutant that can be incorporated into the food chain through contamination of feed materials and complex feeds that require monitoring. There are requirements for the analysis of PCP in feeds and foods (Committee Implementation Regulations (EU) 2015/175), especially due to concerns about guar gum contamination, which does not exceed 0.01 mg / kg for imported foods and feed products. Is shown. PCP.
EN 17362 describes how to determine PCP using liquid chromatography in combination with a triple quadrupole mass spectrometer (LC-MS / MS) in negative ion mode. PCP is extracted from feed material using the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) approach of fatty acid distillates (FAD) and the “reverse” QuEChERS approach of guar gum. For pre-extracted FAD samples, sulfuric acid is used to remove the lipids. Internal isotope standard 13C labeled PCP is added during the extraction phase to ensure good recovery and reproducibility.
This method was validated by joint testing of PCP in compound feeds, guar gum, and fatty acid distillates ranging from 9 µg / kg to 22 µg / kg. The quantification limit (LOQ) for PCP measurements in guar gum, fatty acid fractions (FAD), and animal feed is 10 µg / kg.
EN 17374: 2020 Animal feed: Sample and analytical methods-Anion exchange HPLC-Measurement of inorganic arsenic in animal feed by ICP-MS
Arsenic is more toxic than trivalent arsenate (As + 3) than pentavalent arsenic (As + 5). Arsenic varies greatly depending on the valence of arsenic ions as inorganic and organic compounds. It exists in the form of. Organoarsenic compounds are generally present in pentavalent form, as in the case of arsenobetaine and arsenocholine found in fish meal and oil feeds, which are very unlikely to be toxic.
The requirements for arsenic in feed are set out in Directive 2002/32 / EC, which refers to total arsenic, which distinguishes between inorganic and organic forms of arsenic, as it could not be measured primarily by the analytical techniques available at the time. not.
Recently, the development of new technologies has enabled reliable measurements for quantifying organic and inorganic arsenic in various matrices, a total that may be entirely derived from the following organoarsenic compounds: Instead of measuring arsenic, it is now possible to specifically measure toxic arsenic components. Very low potential for toxicity.
EN 17374 describes how to measure inorganic arsenic in animal feed using anion exchange high performance liquid chromatography combined with an inductively coupled plasma mass spectrometer detector (HPLC-ICP / MS). .. The sample is dissolved in dilute nitric acid and hydrogen peroxide in a warm water bath before injection into HPLC-ICP / MS.
This method has been reported to be applicable in the following animal feed matrix in the range of 0.149 mg / kg to 9.69 mg / kg: rice flour, seaweed flour, fish meal, grass meal, complete feed (ocean-based) and complete. Feed (grain-based)). The mineral feed matrix is not included in the scope of this method.
CEN / TS 17455: 2020 Animal Feed-Sampling and Analytical Methods-Performance Criteria for Single Laboratory-Validated and Ring-Trial-Validated Analytical Methods for Measuring Mycotoxins
Technical Specifications (TS) are approved normative documentation specifications for experimental and / or evolving technologies.
CEN / TR 17455 provides laboratory guidance in designating performance criteria for single-laboratory or collaborative-validated methods of analysis of mycotoxins in feed.
This guide will help you assess the quality of the new European standard method under verification and confirm the quality of previous collaborative trials. To confirm the expansion of the range of already published European standards that apply to concentrations or matrices of other analytical objects. Alternatively, evaluate the relevance of a single validated method. Performance criteria are particularly applicable to methods of measuring mycotoxins.
Regular updates on changes in food and feed legislation are published by government chemists.
Food and Feed Law: Overview of UK Food and Feed Law with Relevant Situations and Changes from July to September 2019-Government Chemist Program Report
CEN renewal affecting chemical measurements April-September 2020
SourceCEN renewal affecting chemical measurements April-September 2020